Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

沥青路面罩面的设计方法

针对国内外罩面或改建设计方法的不足 ,借助于同济大学对新建和罩面改建后的沥青路面使用性能的研究成果 ,将寿命周期费用分析引入罩面设计 ,提出了沥青路面罩面设计方法———基于使用性能和技术经济分析的最佳罩面时机和最佳罩面厚度选择方法 ,并通过实例说明了该方法的应用     

流行装定价策略与价格模型的建立

利用产品生命周期理论对流行装定价策略进行研究,并以流行周期及其所处流行区段为基础构建流行装基准价格模型。通过考察品牌、销售场所、售后服务和市场竞争等因素对流行装价格的影响并探讨各因素权重取值方法,建立流行装市价模型。     

虚拟企业生命周期分析与系统管理

虚拟企业是一种新的合作模式,它可以适应不断变化的市场环境,因此自从它被提出后就受到企业界和学术界的广泛关注,并取得了一系列的研究成果。虚拟企业是由两个或两个以上的独立企业组成的一种临时性网络组织,当期望目标达到后此组织即解散。成功地实施虚拟企业可为企业带来可观的收益,但实施虚拟企业战略也有许多风险。作者在分析虚拟企业生命周期的基础上,提出了虚拟企业生命周期系统管理策略。     

RTK快速求解整周模糊度算法及应用

分析了GPS-RTK测量速度的关键即解算整周模糊度，通过对RTK整周模糊度算法原理的分析，提出了快速RTK求解整周模糊度算法．该算法首先确定未知点的初始坐标，进而得到初始模糊度；然后以初始值为中心建立一个搜索空间，应用最小二乘原理搜索出准确的模糊度值．     

混合热阻条件下广义热机的最优构型及其性能

研究了有限热源条件下工质与热源间传热规律服从混合热阻形式时热机的有限时间热力学性能，讨论了循环的最优构型及最优功率与效率间的优化关系，所得结果对实际热机的设计工作具有一定的指导作用。     

中国亚热带东部丘陵区农田生态系统水循环特征

以水量平衡理论为基础,定量研究中国亚热带东部丘陵区农田生态系统在自然状态下与人类影响条件下的水循环特征,探讨农田生态系统水循环过程与区域性水循环过程之间的差异。结果显示:本区多年平均生态需水量2756.4×108m3,降水过程满足了其中的81%,亏水量534.5×108m3,剩余水量1666.5×108m3;系统中水稻的有效降水量虽然很大,但水分满足率最低;薯类、豆类等作物的有效降水量小,但水分满足率较高。然后分析了历年水旱灾害记录与农田生态系统水循环过程之间的对应关系,以证明结果的准确性。     

一类Bogdanv-Takens系统极限环的唯一性

用Dulac函数法证明一类广义Lienard系统极限环的唯一性，由此唯一性定理导出一类Bogdanv-Takens系统至多存在一个极限环。     

Willis环上脑动脉瘤模型的混沌分析

研究如下的Willis环上的脑动脉瘤模型：x εμx αx γx^3=εFcos ωt。首先阐明了Smale马蹄变换意义下的混沌是Devaney混沌，然后用Melnikov函数方法得到了在两种情况下该模型存在Devaney混沌的条件。